Role of Methylated RASSF1A Gene as a potential biomarker in the diagnosis of lung cancer

Mahmoud, Eman M; Abdulkareem, Ebtsam Sayd; Sayed, Suzan; Salama, Ragga; Elkholy, Maha

doi:10.21608/muj.2022.144129.1109

Role of Methylated RASSF1A Gene as a potential biomarker in the diagnosis of lung cancer

Document Type : Original Article

Authors

¹ PORT SAID UNIVERSIY

² Department of Chest diseases Faculty of Medicine, Aswan University

³ Department of Chest diseases Faculty of Medicine, Assiut University

⁴ Department of medical Biochemistry & Molecular Biology , Faculty of Medicine, Assiut University

⁵ Department of Chest diseases Faculty of Medicine,Assiut University

10.21608/muj.2022.144129.1109

Abstract

The leading reason of cancer-related mortality in the world is Lung cancer. human RASSF1A has been extensively recognized as an indispensable goal suppressor tumor gene. of expression of RASSF1A loss was once shown to take part in the pathogenesis of different human cancers.
Aims: Verify the significance of DNA methylation of the RASSF1A gene as an early detection biomarker for lung cancers.
Settings and Design: randomized managed scientific study
Methods and Material: This finds out about included 50 folks in Aswan and Assiut University Hospitals, divided into two corporations along with 30 sufferers with lung cancer and 20 aberrantly wholesome heavy people who smoke as a management group. Both corporations were subjected to clinical examination, imaging modalities, activities laboratory investigation, and blood patterns for methylation-specific PCR and DNA extraction in addition bronchoscopy and histopathological examination have been executed on the group of lung cancer patients.
Statistical analysis used: SPSS 20.0 for home windows and MedCalc thirteen for home windows
Results: RASSF1A gene was hypermethylated by way of 64.8 folds in lung cancer sufferers extra than in the manage group. There used to be a statistically considerable difference between those with methylated shape and those with an un-methylated form of the RASSF1A gene in the two studied groups, the frequency of methylation used to be greater in patients with lung cancer than in the control crew. Conclusions identifying the methylation fame of the RASSF1A gene may be beneficial as a molecular biomarker in analysis and screening for lung most cancers.

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