Protective impact of Hesperidin in hyperthyroid-induced Cardiomyopathy in rats

Khodir, Suzan A; Abd El-aziz, Noha; Emam, Reem M; Elmalky, Heba Ashraf; Abdalraouf, Mai M; Shahien, Mohamed Awad; Ahmed, Reem Mohammed; Abd-Elhamid, Tarek H; Abd Elmaksoud, Rasha Arafa; Elmorshdy, Shorouk E. M.

doi:10.21608/muj.2025.366265.1211

Protective impact of Hesperidin in hyperthyroid-induced Cardiomyopathy in rats

Document Type : Original Article

Authors

¹ Medical Physiology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt.

² Anatomy and Embryology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt

³ Medical Physiology Department, Faculty of Medicine, Mansoura University, Mansoura, Egypt

⁴ Endocrinology unit, Internal Medicine Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt

⁵ Cardiology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt

⁶ Clinical Pharmacology Department, Faculty of medicine, Damitta University, Egypt

⁷ Medical Biochemistry and Molecular Biology Department Faculty of Medicine (Girls), Al-Azhar University

⁸ Histology and Cell Biology, Faculty of Medicine, Assiut University, Assiut, 71515, Egypt. Histology, Faculty of Medicine, Aqaba Medical Sciences University, Aqaba, 77110, Jordan

⁹ Pathology Department, Faculty of Medicine, PortSaid University, Egypt.

10.21608/muj.2025.366265.1211

Abstract

Background: Higher risk of cardiovascular problems is linked to hyperthyroidism. The citrus bioflavonoid hesperidin (HSP) has cardioprotective, anti-inflammatory, and antioxidant impacts.
Aim of the study: to illustrate the possible underlying processes and cardioprotective impact of HSP in cardiomyopathy caused by hyperthyroidism.
Material and methods: Thirty male albino rats were split into three groups: Hyperthyroid, Hyperthyroid+HSP, and control (10/group). After four weeks rats were subjected to ECG evaluation, LVW/ tibial length, Serum TSH, Serum total T3, T4, Serum LDH, Serum CK-MB, Cardiac MDA, Cardiac SOD, Cardiac TNF-α, Cardiac IL-6, Cardiac genes expression of ANF, Caspase-3 and NF-kB evaluation. Furthermore, cardiac histological and immunohistochemical analyses were performed.
Results: There was dramatically elevated heart rate, LVW/ tibial length, serum tT3, serum tT4, serum LDH, serum CK-MB, cardiac MDA, cardiac TNF-α, cardiac IL-6, cardiac ANF gene expression, cardiac Caspase-3 gene expression and cardiac NF-kB gene expression alongside a marked reduction in duration of PR interval and QT interval, serum TSH and cardiac SOD of hyperthyroid group compared to control. HSP dramatically ameliorated hyperthyroid-induced cardiac alterations generated by hyperthyroidism.
Conclusion: In hyperthyroid-induced cardiomyopathy, HSP has a cardioprotective effect by enhancing ANF levels and regulating the heart's anti-inflammatory, anti-apoptotic, and antioxidant mechanisms.

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