Document Type : Original Article
Authors
1
Medical Physiology Department, Faculty of Medicine Ain Shams University, Cairo, Egypt. Medical Physiology Department, Faculty of Dentistry, Misr International University, Cairo, Egypt
2
Medical Physiology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt.
3
Anatomy and Embryology Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt
4
Neuropsychiatry Department, Faculty of Medicine, Menoufia University, Shebin El-Kom, Menoufia, Egypt.
5
Clinical Pharmacology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt
6
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Benha University, Egypt
7
Pathology Department, Faculty of medicine Mansoura University, Egypt
8
Zoology department, Faculty of Science, Menoufia University.
9
Medical Physiology Department, Faculty of Medicine, Cairo University, Cairo, Egypt
Abstract
Introduction: Autism spectrum disorder (ASD) is linked to stereotypical behavior and poor social skills. Quercetin (Qrct) has been shown to possess anti-inflammatory and antioxidant impacts.
Objective: to demonstrate the underlying processes and the neuroprotective impact of Qrct in VPA-induced ASD.
Material & methods: Thirty male Wister albino rats were split into three: control, VPA, and VPA+ Qrct. Following neurobehavioral testing, the rats were sacrificed, and the cerebellar gene expression of TLR4 and NF-kB was evaluated together with the measurements of cerebellar MDA, SOD, TNF-α, IL-6, IL-10, BDNF, and serotonin. Cerebellar and histopathological immunoreactions for Bax and NF-kB were performed.
Results: While the VPA group's number of crossing slots in OFT, time of central crossing in OFT, cerebellar SOD, cerebellar IL-10, cerebellar BDNF, and cerebellar serotonin were dramatically decreased than those of the control, the VPA group's rearing frequency in OFT, time in the open arms of EPM, cerebellar MDA, cerebellar TNF-α, cerebellar IL-6 and cerebellar TLR4 and NF-kB gene expression dramatically increased than those of the control. Additionally, the VPA group's NF-kB and Bax cerebellar immunoreaction were dramatically increased than those of the control. Qrct significantly enhanced ASD caused by VPA.
Conclusion: Qrct reduced VPA-induced ASD by down-regulation of the cerebellar TLR4/NF-kB pathway as well as anti-oxidant, anti-inflammatory, neurotropic, and antiapoptotic pathways.
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